I changed over a well established 55 gallon. I took everything out...LR, CC, water, fish, inverts, everything. Put is buckets. Took the CC and put it in mesh media bags that you can get at the LFS. I put 100 pounds of Southdown Playsand from Home Depot in and 10 pounds of real LS from my LFS in...not the bagged stuff...the bagged stuff only has bacteria, not pods, etc. I then put the LR, fish, water, and inverts back in, along with the 2 bags of CC that was in there. The bacteria from the CC will migrate to the sand. I took one bag out after 3 weeks and the other after 4 weeks. No spike, no cycle. The tank was crystal clear in less than 12 hours.