That's a lot to consider and think about thank you. I will have to start testing for some of those things. All I can tell you at this point is that I had mushroom polyps, mushrooms, richordea, Kenya tree, and green star polyps. Forgive me if any of these names are wrong. I had most if not all of those for the better part of 2 or 3 years. They were all doing well, I was propogating most of them, some were spawning on their own. I got a new light fixture corallife 48" t5 about a year ago. All was well, haven't added any fish, no fish have died, besides a clown that took the jump. I added the protein skimmer and sump and that was about the time that all at once, my richordea died in the matter of days, my red mushrooms disappeared, my star polyps are alive but struggling, my Kenya tree seems to be fine, seems a little "limp" but it is still spawning. My red mushrooms seem to keep coming back and then disappearing. I realize that there is quite a few things that I need to be checking. But overall my question is can a protein skimmer contribute to these types of problems. And from here I will start getting some testing supplies as I can afford.