In my best estimation - yes
The top off water in the holding tank should be heated and aerated with a powerhead .. ESPECIALLY if it's RO or RO/DI water that you are making at home.
Allowing dissolved CO2 gas in the freshwater to reach equalibrium with the CO2 gas in the atmosphere ( room ) is an important step.
Heating and aerating the top off water would be a great thing to do
