Yes, No, and Maybe. Different organisms are affected by different things and too different degrees by the same thing, so its really hard to say what will be killed and what will not. But if you kill off all your bacteria, then ammonia levels will increase, which could then kill off everything else ~ you see? Its complicated. Thats why I said "better safe than sorry". Aquarium safe silicon really shouldn't be that more expensive then regular, why take the chance
? As for curing time, the tube should tell you, but if you use the non aquarium stuff, I would give it an extra day or so, just to make sure.