RO water should not be used either. You should really only use distilled water to calibrate your refractrometer. You can make your own by boiling tap water, preferably in a tea kettle. Hold a coffeee mug upside down over the steam. I use a coffee mug because it has a built in handle. When the steam condenses back into water, it will be distilled. You only need a few drops to calibrate your refractometer. You can easily get that much and then some by this method.