it depends on whats in ur tank now. if u have fish/inverts in there, putting LS in may cause a new or "mini" cycle because there will be die off from the LS. In that case, u would want to cure it seperately. If there's nothing in ur tank but LR, its fine to put it in.
Depending on how much ur adding, u may or may not see an ammonia spike. That will depend on how much bioload ur existing bio filter can handle.