1 - Fill test tube to 5ml line.
2 - Add 7 drops of reagent #1. Shake well to mix. Avoid skin contact.
3 - Shake reagent #2 bottle for 5 seconds and add 3 drops. Shake well to mix.
4 - Add 1 drop of reagent #3. Insert cap and shake well to mix. A pink color should be produced. If, after 1 drop, the color changes from light pink to violet, the calcium is below 20mg/l.
5 - If color remains pink, continue to add 1 drop at a time and shake. Count each drop and stop when the color changes to violet.
6 - Multiply total number of drops by 20 to determine calcium in mg/l.
7 - After use rinse test tube.
Good luck!