Um y is ur ammonia at .25 it should be at zero and ur purple shrooms arent reacting as fast to the 40-50 ppm nitrate level because they are more hardier, but eventually with time they will die off. for a reef u should really keep them below 5 ppm. 40-50 ppm nitate is even bad for fish. You should bring that down, and are u sure that ur doin water changes with RO/DI. If You do tell me where you got it from and if its bottled already tell me wat is says on the label. Thanks Oh and u should really raise ur water gravity to 1.024-1.026, its better for fish in the long run and u should keep reef tanks in that range too.