I think before you can really answer this question, you first need to determine what your goal is...
If you want to use the sand bed as a denitrafication (reduce nitrates-I can't spell) tool, then I think it's impossible to use only 3 inches b/c more than likely oxygen will provide respiration to the batcteria...if all you want to do is develop a sand band that converts amm. and trites to trates, then 3 inches if fine.
I think this poll needs some more qualifications, like what are your really trying to achieve.
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