I'm not quite sure what Amquil does. I was told to let the tank "mature" by itself. Test your ammonia, nitrite and nitrate on a daily basis and graph your results. When you see the results decreasing toward zero, then your nitrogen cycle is complete"ing". As for the bacteria, you definitely need to seed your tank, especially since you did not use lr or ls. Bacteria is what stimulates the nitrogen cycle. I personnally do not know anything about bottled bacteria, (I used ls), maybe some other hobbyists have experience with this. Best bet is to use the SEARCH