newone
Member
sturun3@comcast.net
lost instructions
- Thread starter newone
- Start date
sturun3@comcast.net
viper_930
Active Member
Salifert Calcium Test
1. Add with the syringe 2 ml. of water in the test vial. For a lower resolution and more tests per kit add 1 ml. instead of 2.
2. Add 1 level spoon of Ca-1 (low resolution approx 1/2 spoon). Do not mix it yet.
3. Add 8 drops of Ca-2. The low resolution mode requires 4 drops.
4. Put the plastic tip firmly on the 1 ml. syringe. And draw into the syringe the Ca-3 reagent (ensure that the end of the plastic tip is constantly sumbersed in the Ca-3 reagent) till the lower end of the black part of the piston is exactly at the 1.00 mark. There will be some air present just below the piston. This is the air which was present between the end of the plastic tip and the piston. This will not influence the test result.
5. Add dropwise with the 1 ml. syringe the Ca-3 reagent to the water in the test tube. Swirl after each drop a second or two. Continue with this until the color changes from the pink-red to a clear blue color.
6. Hold the syringe with the top facing upward and read the position of the, now upper end, of the black part of the piston. The syringe has graduations of 0.01 ml. Read the calcium value from the table or calculate as follows.
ppm Ca = (reading in step 6) x 500
Reading in step 6..........ppm Ca
0.00..............................500
0.10..............................450
0.20..............................400
0.30..............................350
0.40..............................300
0.50..............................250
0.60..............................200
0.70..............................150
0.80..............................100
0.90..............................50
1. Add with the syringe 2 ml. of water in the test vial. For a lower resolution and more tests per kit add 1 ml. instead of 2.
2. Add 1 level spoon of Ca-1 (low resolution approx 1/2 spoon). Do not mix it yet.
3. Add 8 drops of Ca-2. The low resolution mode requires 4 drops.
4. Put the plastic tip firmly on the 1 ml. syringe. And draw into the syringe the Ca-3 reagent (ensure that the end of the plastic tip is constantly sumbersed in the Ca-3 reagent) till the lower end of the black part of the piston is exactly at the 1.00 mark. There will be some air present just below the piston. This is the air which was present between the end of the plastic tip and the piston. This will not influence the test result.
5. Add dropwise with the 1 ml. syringe the Ca-3 reagent to the water in the test tube. Swirl after each drop a second or two. Continue with this until the color changes from the pink-red to a clear blue color.
6. Hold the syringe with the top facing upward and read the position of the, now upper end, of the black part of the piston. The syringe has graduations of 0.01 ml. Read the calcium value from the table or calculate as follows.
ppm Ca = (reading in step 6) x 500
Reading in step 6..........ppm Ca
0.00..............................500
0.10..............................450
0.20..............................400
0.30..............................350
0.40..............................300
0.50..............................250
0.60..............................200
0.70..............................150
0.80..............................100
0.90..............................50