I mixed the water in a 35g bucket. After it mixed for 24hrs then I put about 4 inches of the mixed water in the tank so that when I put the sand in it decreased the die off. After adding the sand then I put the plate in and added the water a gallon at a time till it was almost full, let it set for a couple of hours, then turned on the HOB. The next morning the water was clean. Then I added the rock and started the cycling with a shrimp wrapped in a piece of nylon.