Two weeks ago, i moved all my fish from my apt& school in WV to my summer job in Western MD. i had a Tomato Clown, a royal Gramma & a Saddleback clown in my home tank. i removed them, put them in water straight from my tank, & a cooler at noon. They sat in my car all afternoon while i cleaned up my classroom & got the rest of my fish from my classroom. By the time i arived up at their "summer home" it was after 8 p.m. I was pleasantly surprised to see that all 3 of the fish that had sweltered in my car all day had survived w/ nary a glitch. The only problem i had ws that my saddleback hid in the corner for about 3-4 days. But pack like the previous post, w/ Plenty of O2 & in a cooler. As for travel by plane, i'm sure that's possible also, since that's how they're shipped across the world. There's probably no need to worry about their ears popping.