My dad and I used to transport small fish, inverts, aneomies, and urchins that we collected from S. Carolina and Florida to northern Indiana. The drive was between 16-20 hours. It was very rare that we lost anything. We always used large coolers, oxygen tablets, and brought multiple containers of ocean water with us. We would stop every 3-4 hours and do small water changes. Usually we drove a van and only turned it off to get gas--wanted to maintain a constant temperature. It was alot of work, but we sure had a great time spending the week collecting thing and then watching them grow in our 180 and 65 gallon tanks--I sure learned alot from that when I was a kid and had a lot of great memories. Often we would trade our lfs for things as well. My favorite was a 1/2" porcipine puffer that we had for over 10 years--finally our local zoo took him because he outgrew our tank. We just did alot of research while collecting things, didn't want to take anything with us that was inappropriate, difficult to keep, outgrown the tanks..etc.