Originally posted by Darknes
Yes, but you will still have a spike since the bacteria isn't used to having extra bioload..just like when you add a new fish to a cycled aquarium, there still might be a jump since the bioload increases and takes a while to accomodate it.
It would however, be a much smaller spike because the bacteria is already there like you suggested
The Bacteria multiply +- every 20 minutes.
Over the course of a day.... 1 Million bacteria added, become over a trillion. Since the Ammonia level in the tank when you start is, by definition, ZERO, and you just put in a couple of tiny fish or two, you would never produce ammonia fast enough to spike. In fact, to the opposite point, the ammonia becomes the "limiting factor" in the growth of the bacteria culture. It can't reproduce as fast as it would like to without the ammonia there...so it's actually being slowed down substantially. This is a good thing, of course. If you dumped in ammonia on day one, you'd produce too many bacteria, which, with the ammonia then withdrawn, would all die out.
Steve