Originally Posted by
PEZenfuego
http:///forum/post/3133796
They would not. Even with no source of seed, the tank would be able to cycle in the matter of a month to a month in a half. Most people don't realize this, but LIVE rock is not essential for cycling a tank.
If can't stress this enough, if anyone is to do this, make sure that your source of ammonia is stable (ie not a raw shrimp, but rather a bottle of pure ammonia) and that the "pure" ammonia is free of surfactants, dyes, etc. The label can only have 2 ingredients and no more than two. Those being ammonia and water. Anything else and the experiment would be worthless.
If you shake the bottle and foam is created...the experiment is worthless. If the color of the bottle is anything other than clear...the experiment is worthless. If you don't measure out the same amount of ammonia for both tanks...you are worthless.
Of course to start off you would want to add about 5 ppm to each tank. You can do this by first adding about 1 teaspoon to a neutral container of water (this container of water will not be either of the test subjects and the amount will have to be known. Ie, a one gallon container will do well). Keep adding this ammonia and testing until you get it up to 5 ppm. Once that is done take note of how many teaspoons per gallon you need and multiply that to come up with the amount of ammonia to add for the two test tanks.
I really don't follow you. Of course, a tank will cycle just using the bacteria available. But, different media (rubble & bio-balls) will probably cycle at different rates. If a tank does not have an active and stable culture of aerobic bacteria; comparisons of ammonia reduction are worthless. A new, dry piece of LR rubble or a new bio-ball will not reduce ammonia at all. I guess this is just a long way of saying that a tank must be cycled (in any manner) to produce valid results. (IMO) Also, other media capable of holding aerobic bacteria (substrate, filter pads, carbon, etc) would also skew results. Bio-load is also a factor. I would think there are many other variables that influence rate of ammonia reduction; like PH, temp, SG and anything else that could affect bacteria activity.