Bang you are right enzyme actively is greatly affected by pH concentrations, especially allosteric enzymes. Just like our immune system enzymes have an optium pH to they function at, slight moderiations in pH will affect the concentration of enzyme that is available to bind substrate and allows for "fine tuning" of activity. Most things like amino acids, proteins, enzymes, etc are bound by weak van der Waals forces and changing the pH of a system is enough to denature (break the bonds) them. I am not really to sure how probable it would be that anemone's rely on enzymes from the ocean, firstly enzymes are living not an inorganic nutrient, so they exist in organisms. I guess secondly i would have to think researchers would have discouvered this required nutrient unless its trace and exists at a very low concentration but then what would the probability of a particular anemone being able to uptake this nutrient ? and don't they live hundreds of years in the ocean. I would agree that there has to be some parameter missing from our closed aquatic systems but would be declined to believe it would be an enzyme. Everyone has mutations even you and i almost always they are not expressed, example; the deamination of cyctosine to uricil occurs in 1/10 x 10^7 base pairs per 24 hour period, but never builds up due to enzyme repair. If a mutation is magnified enough it leads to an adaptation. Adaptations begin with gene mutations that usually have no effect on an individual species. However, if the animal reproduces, mutations have a small chance of passing forward and increasing in intensity When these favourable adaptions occur, they alter the environment around them (including other life, only a particular species can fill a particular ecological niche). The adaptation can affect the sensory organs, behaviour, mental capacity, gestation period, size, etc.. This initial succesful adaptation changes the rules of the game (like moving your pawns sideways in chess). Other animals either die off, or adapt themselves. The complex interplay between collective and competitive actions is lost; a succesfully adapted animal enhances the survial odds of its species in its ecosystem. I have no idea how much research has been put into studying the biochemistry of anemones but if captive aneomnes have a defficiency of a particular enzyme i would believe it would be the result of a particular nutrient of co-factor not present.
thanx for the good info. but do you think there might be different results if you didnt plumb into the lagoon, i belive it may be to stable of a system for an experement like this. 
