al&burke
Yes use floss & sponge only......u can grab a couple square inches from a main floss filter (if used) that is already saturated with nitrifying bacters to supplement cycle in Qtine, but only do this once, and at the beginning of Qtine....Primarily - it's partial waterchanges every 2-3 days to keep nitrates/nitrites/ammonia in check. ..Qtine tanks can/will be cloudy and thats OK, as long as nitrates/nitrites/ammonia do not reach stress levels.....Test these levels daily .....NO carbon - and do not use ANY sand/liverock/aragonite/reef gravel in hospital tank (Qtine copper treatment). All the standard reef type substrates/rock WILL absorb copper meds, and make stable ppm treatments difficult, as these things can absorb copper meds and reduce the treatment value in HT,,,then they can leach the absorbed copper back later if any of these items go back into any main system and wipe out inverts.....A Qtine copper treated tank serves only one purpose - kill parasites without killing the fish, until fish are healthy enough to remain in that Qtank without copper, and by that time, the main has been fallow long enough that no parasites remain....
The complete life cycle (including dormant stages) of marine Ich is 4 weeks in a contaminated main tank with no fish...and thats the minimal time the main should be fallow (no fish). With copper treatments running at least 3-4 weeks in Qtine, and if no signs of Ich remain, I usually totally breakdown and very dutifly clean out my Qtine tank, flushing everything with hot water, and re-insert the fish for 2 more weeks with 100% new water & filters & no copper, just to be sure they are in a parasite free state in Qtine...only then do I put them back into a main, and that's now 6 weeks from outbreak in the main........If steps are rushed, you could have to repeat a Qtine cycle and now thats 2-3+ months, and most fish cant take copper meds that long......it's best to clear the problem on the first try with fish in treated Qtine 4 weeks, then 2 weeks no meds, but still in Qtank - then back into main after 6 weeks fallow....
Only thing to do then is monitor fish for signs of re-infection in main, and never ever put anything from aonther system, including live foods - in your tanks....
my $0.02